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With pulses of light in the right part of the brain at the right frequencies, Brown University scientists induced rats to behave as if things they’d seen before were novel and things they’d never seen were old hat.
In experiments, researchers introduced rats to images and then showed them both the old and new ones. They found they could control each rat’s sense of whether images were old or novel. Photo Credit: Burwell lab/Brown University
“In a sense we were trying to ‘write in’ novelty and ‘write in’ familiarity,” said Rebecca Burwell, professor of cognitive, linguistic and psychological sciences at Brown and corresponding author of the new paper in the Journal of Neuroscience.
Burwell’s research group was interested in how behaviors associated with recognizing novelty and familiarity arise in the brain. The field already understood what area in mammal brains is important (the perirhinal cortex or PER), but the literature only contained hints that the PER might work based on brainwave patterns there.
“We knew very little about how information about novelty or familiarity is coded or transmitted,” said Burwell, who is affiliated with the Brown Institute for Brain Science.
Now Burwell and her co-authors have demonstrated the exact patterns precisely, achieving such facility of control that they could consistently compel different groups of rodents to do the opposite of what they normally would.
The rats spent most of the time in a simple maze akin to two stalls divided by an opaque wall. Visual images could be presented at the front of each stall. They had days to become acclimated to this environment. Testing sessions entailed introducing a rat to the same black and white image in each stall. After a five-minute respite back in the home cage, the rat returned to the maze to encounter one of three cases: One stall showed a new image and one showed the old; both stalls showed something new; or both still showed the original image.
Just like a person, a normally behaving rat will spend more time looking at a new image than an old one. This is a sign that the people and rats recognize the old item. But in these experiments, Burwell’s team, including lead authors Jonathan Ho and Devon Poeta, altered some of the rats’ behavior by manipulating the brain using optogenetics. That technology allows scientists to control the activation of neurons in any area of the brain that they expose to specific colors of visible light. Burwell, Ho, and the group used this capability to alter activity in the PER by pulsing the light into that part of the brain at different frequencies.
To create different experimental conditions, the researchers would sometimes flicker the lights at different frequencies when the rats entered a stall and looked at the image. In some cases, for instance, if a rat approached a novel image they’d flicker the light at frequencies between 10 and 15 hertz (cycles per second). Sometimes when the rat approached a familiar image they’d flicker it between 30-40 hertz.
During the experiments, researchers including Poeta would painstakingly time how long rats gazed at every image. The human observers did not know whether the image was novel or familiar and were similarly blinded to whether and when the brains of the rats were stimulated with optogenetic light.
Over many trials with two independent groups of rats, the researchers’ subsequent analysis showed the same statistically significant patterns. Without optogenetic stimulation, rats would give longer looks at novel images than at familiar ones. With 30 hertz stimulation, rats would look at familiar images longer than they would look at the same familiar image without such stimulation — as if the familiar images were novel. Stimulation at 11 hertz had the opposite effects by reducing the time the rats invested in novel images to the point where they seemed to regard them as familiar.
“This suggests that the 30-40 hertz stimulation of caudal PER does simulate the effects of novelty on exploratory behavior and that the 10-15 hertz stimulation does simulate the effects of familiarity,” the authors wrote.
Other frequencies outside these ranges did not show the same behavioral effects. They confirmed that 30 hertz seems to be optimal for inducing novelty and about 11 hertz works well for inducing familiarity.
When the researchers looked at activity at the cellular level in the rats’ brains, they saw that individual cells stimulated by the optogenetics would increase firing rates in the PER network and that many cells fired in sync with the optical flicker frequency.
The team’s new research has continued to explore these networks on a grander scale, incorporating the PER’s interactions with the prefrontal cortex, where executive decisions are made. That allows them to better understand when and why a rat might choose to explore a novel image or to defer that behavior.
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The above post is reprinted from materials provided by Brown University.
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